Acute Leukemias of Ambiguous Lineage

Acute leukemias of ambiguous lineage (also known as acute leukemias of undetermined lineage, mixed phenotype acute leukemias, mixed lineage acute leukemias, and hybrid acute leukemias) are types of acute leukemia in which the morphologic, cytochemical, and immunophenotypic features of the blast population do not allow classification in myeloid or lymphoid categories; or the types have morphologic and/or immunophenotypic features of both myeloid and lymphoid cells or both B and T lineages (i.e., acute bilineal leukemia and acute biphenotypic leukemia).

Epidemiology

These rare leukemias account for less than 4% of all cases of acute leukemia and occur in all age groups but are more frequent in adults. Clinical features include symptoms and complications caused by cytopenias, i.e., fatigue, infections, and bleeding disorders.

Possible causes

Similar to other acute leukemias the aetiology is unknown. Environmental toxins and radiation exposure are possible causes.

Morphology

Acute biphenotypic and acute bilineal leukemia may present either as one of the subtypes of AML or with features of acute lymphoblastic leukemia. in some cases the blasts are morphologically undifferentiated.


Example bilineal leukemia, two distinct blasts present.	Example bilineal leukemia, two distinct blasts present.	Example bilineal leukemia, two distinct blasts present.

Immunophenotyping

The CD45 vs SSC gating dot plot identifies a blast population which is CD45 dim/negative and SSC low.

Example gating dot plot from a case of biphenotypic acute leukemia.

Below are example dot plots gated on the above blast poulation of a biphenotypic leukemia case



The above dot plots show CD13 positive and cytoplasmic CD3 positive.	CD34, CD117, MPO and CD34 are positive.	TdT, CD1a and CD34 are positive.

The differential diagnosis includes myeloid antigen-positive ALL or lymphoid-positive AML (from which biphenotypic acute leukemia should be distinguished) and minimally differentiated AML (from which undifferentiated acute leukemia must be distinguished).

Lineage Scoring

The European Group for the Immunologic Characterization of Leukemias has developed a scoring system based on degree of lineage specificity of each antigen. two or more points in two categories are needed to diagnose a biphenotypic leukemia.

Score	B-Lymphoid	T-lymphoid	Myeloid
2	cyCD79a	CD3(surface/cy)	MPO
	cyIgM	TCR
	cyCD22
1	CD19	CD2	CD117
	CD20	CD5	CD13
	CD10	CD8	CD33
		CD10	CD65
0.5	TdT	TdT	CD14
	CD24	CD7	CD15
		CD1a	CD64

Other relevant tests

Genetics: Cytogenetic abnormalities are observed in a high percentage of bilineal and biphenotypic leukemias. [Approximately 33% of cases have the Philadelphia chromosome, and some cases are associated with t(4;11)(q21;q23) or other 11q23 abnormalities. In general, the prognosis appears to be unfavorable, particularly in adults; the occurrence of the translocation t(4;11) or the Philadelphia chromosome are especially unfavorable prognostic indicators.

Sub-classification

Acute bilineal leukemia

Defined as two separated blast populations, one myeloid and the other lymphoid, or rarely one B cell and one T cell. Acute bilineal and biphenotypic leukemias are less than 4% of acute leukemias. They often have a poor prognosis. DD: ALL or AML with aberrant antigen expression.

Acute biphenotypic leukemia

Less than 4% of acute leukemias. Also called acute mixed lineage leukemia.A single population of blasts expresses myeloid, and usually either B or T cell antigens; expression of B & T or B, T & myeloid antigens is rare. May derive from primitive stem cell with capacity to express markers of both myeloid and lymphoid lineage. They can be either de novo or therapy related, and often have a poor prognosis.

Flow Diagnosis

References

http://www.ncbi.nlm.nih.gov/pubmed/7564526