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We have many pages on the wiki for different diseases in which flow cytometry plays a role in diagnosis. Each page gives a brief description of the disease as well as the key elements in its flow cytometric diagnosis and evaluation. Each case also has an analysis protocol attached containing all the raw data and gating used to analyze the case. Every analysis has detailed annotations beside each plot describing what the plot is used for and key plots that are critical for diagnosing the particular disease are highlighted. An example can be seen (in PowerPoint format) here.
However, PowerPoint (or PDF) is not enough if you really want to learn how to analyze a case. You need to be able to move the gates, markers and quadrants. You should practice changing the plots around to really explore the data. That is why most cases are given in FCS Express format, although there may be cases in PDF and PowerPoint as well. You can download the analysis for free and view/manipulate it using the free FCS Express Reader. We encourage you to download the analysis and practice manipulating the gates to learn the strategies real experts use for evaluating cases.
Since this is a community effort, we want you to contribute. If you see something on one of the pages that you wish to add, or make an explanation clearer, please feel free to do so. Also, if you have examples of cases that we have not listed, or additional cases for conditions that are listed already, please feel free to contribute a case. Some guidelines for contributing cases are listed here
If you are a beginner, please visit our Self-Guided Learning page. The learning page will present the cases in order of complexity, so if you are learning how to do clinical flow, we would suggest you start there.
Case Listing By WHO Criteria
- Acute Leukemias of Ambiguous Lineage
- Acute Lymphoblastic Leukemia (ALL) - General Information
- Acute Myelogenous Leukemia (AML) not otherwise categorized - General Information
- Acute Erythroblastic leukemia (M6)
- Acute Megakaryoblastic Leukemia (AMKL) (M7)
- Acute Minimally Differentiated Leukemia (M0)
- AML (Acute Myelogenous Leukemia with maturation) (M2)
- AML (Acute Myelogenous Leukemia without maturation) (M1)
- AMML (Acute Myelomonocytic Leukemia) (M4)
- AMoL (Acute Monoblastic/Monocytic Leukemia) (M5)
- Acute Myelogenous Leukemias with Recurrent Cytogenetic Abnormalities
- AML with multilineage dysplasia
- Mature B-Cell Neoplasms (general)
- Mature T-Cell and NK-Cell Neoplasms (general)
- Myelodysplastic Syndromes (MDS)
We would like to acknowledge Teri Oldaker for her hard work in making analysis templates that are ideal for teaching flow cytometry and Dr. Mark Shenkin for providing case data.